bioRxivJClub
In vivo imaging of autophagy dynamics in the intact mammalian brain using a fluorescence lifetime-based sensor
bioRxiv · · Preprint · Open access
Maman, Ando + 22 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Faster autophagic vesicle trafficking was resolved in distal versus proximal dendrites of layer 2/3 cortical excitatory neurons in the intact mouse brain, linked to sensory experience.
- Why it matters: Autophagy’s spatial and temporal organization in intact neural circuits is hard to measure, limiting understanding of how it supports neuronal development, plasticity, and metabolism.
- What they did: The authors built an in vivo imaging method using a pH-sensitive fluorescence lifetime sensor plus morphological labeling, validated with pharmacological, genetic, and ultrastructural tests.
- The result: In a newly generated transgenic mouse line, they mapped compartment- and cell-type-specific basal autophagy across excitatory neurons, interneurons, astrocytes, and oligodendrocytes, enabling circuit-level studies in development, plasticity, and disease.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.
- Open access