TRAP complex is essential for coronavirus replication and glycoprotein biogeneses.

TRAPγ is essential for productive infection by porcine hemagglutinating encephalomyelitis virus (PHEV), and its depletion suppresses viral replication while disrupting canonical replication organelle formation.

• Why it matters: Coronaviruses must coordinate ER remodeling for replication organelles with production of heavily glycosylated membrane proteins, but the host factors linking these processes are unclear.
• What they did: In a genome-wide CRISPR-Cas9 screen, TRAPγ was identified as required for PHEV infection; TRAP subunits were then shown to be selectively engaged by viral glycoproteins, and signal-sequence hydrophobicity reduction impaired glycoprotein production and N-glycosylation.
• The result: TRAPγ depletion amplifies innate immune signaling, and OST inhibition recapitulates glycoprotein and targeting defects, supporting a sequential SRP–TRAP–OST axis for viral ER co-translational translocation.

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