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In vivo-compatible spatial multi-omics via hydrogen peroxide-independent APEX2 labeling.
Nature Chemical Biology · · Journal Article
Chen, Guo + more
Abstract ↗AI summary
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Hi-APEX enables hydrogen peroxide-independent APEX2 proximity labeling, and in tumor xenografts and hippocampal neurons it supports direct protein and RNA spatial multi-omics.
- Why it matters: Hydrogen peroxide–dependent APEX2 disrupts redox signaling and blocks live-animal use, limiting spatial multi-omics of redox-sensitive biology.
- What they did: The authors developed Hi-APEX using a clickable tetrazine-phenol probe and showed APEX2 catalyzes TP labeling without H2O2, then benchmarked mitochondrial matrix and dynamic secretomes.
- The result: Hi-APEX outperforms traditional APEX for capturing redox-sensitive processes like stress response and ferroptosis, enabling discovery of authentic stress granule components and mGPX4 interaction networks, including TRMT61B.
The findingWhy it mattersWhat they didThe result
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