Cell surface display for nutritional chemicals: Strategies, mechanisms, and evaluation methods.

Cell surface display technology anchors heterologous proteins on microbial cell surfaces using membrane-anchoring fusions, improving biocatalysis efficiency without cell lysis or protein purification.

• Why it matters: It addresses the need to present enzymes to substrates in a more practical way for industrial biotechnology, while also clarifying how to regulate, anchor, and evaluate such systems.
• What they did: This review systematically covers host microorganisms and summarizes strategies to raise display efficiency via transcriptional regulation, protein secretion, membrane anchoring, and catalytic processing, along with evaluation methods.
• The result: It links these design and assessment frameworks to applications producing functional sugars, functional lipids, nucleotides, and other value-added nutritional compounds, supporting rational deployment in industrial settings.

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