CRISPR-Cas-based live cell imaging of genome dynamics.

Nature Reviews Genetics · 1 Aug 2026 · Review

Zhu, Moerner + 1 more

The abstract is read at the publisher; the summary is JClub's.

CRISPR-based live-cell imaging enables real-time visualization of specific genomic loci and transcripts in living cells using customized guide RNAs and nuclease-deactivated Cas effectors.

Why it matters: Understanding genome 3D architecture and dynamics is crucial for regulation of genome stability, transcription, and cellular function, but requires real-time, locus-specific observation.
What they did: The work summarizes CRISPR live-imaging development and highlights compatible 3D microscopy and analysis approaches that provide spatiotemporal resolution for chromatin communication.
The result: These methods are elucidating chromatin biophysical properties, linking dynamic enhancer–promoter interactions directly to transcription, and informing applications to study genome dynamics in disease.

AI summary of the abstract · may contain errors · not medical advice · read the paper.

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