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RhoA effectors ROCK and SLK/LOK oppositely regulate VE-cadherin mobility at endothelial junctions
bioRxiv · · Preprint · Open access
Nelson, Lin + 5 more
Abstract ↗AI summary
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In cultured human umbilical vein endothelial cells, SLK/LOK inhibition selectively decreases VE-cadherin mobility within junctions, opposite to ROCK inhibition, which increases mobility both inside and outside junctions.
- Why it matters: Contractile RhoA signaling shapes VE-cadherin dynamics, but how its parallel effectors ROCK and SLK/LOK differentially regulate cadherin movement at endothelial junctions was unclear.
- What they did: Using single-molecule tracking of Halo-tagged VE-cadherin in HUVEC, the authors quantified diffusion inside versus outside junctional regions and tested ROCK inhibition with Y-27632 and SLK/LOK inhibition.
- The result: ROCK inhibition increased VE-cadherin mobility globally, while SLK/LOK inhibition reduced mobility only at junctions and increased reticular junctions, implying SLK/LOK affects cadherin dynamics via junctional architecture.
The findingWhy it mattersWhat they didThe result
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