NDE1 Localizes to the Subdistal Appendages to Maintain Centrosome Integrity and Microtubule Organization

Endogenous NDE1 forms a ring-like structure at the subdistal appendages, positioned between CEP128 and NIN layers, in mouse embryonic fibroblasts and human RPE-1 cells.

• Why it matters: Subdistal appendages are layered to organize microtubules and coordinate centrosome functions, yet NDE1’s nanoscale placement and architectural role remain unclear.
• What they did: Using expansion microscopy and STED super-resolution imaging, the study mapped NDE1 at centrosomes and tested how ODF2, CEP128, CEP170, and NDE1 depletion affect subdistal appendage assembly and microtubule organization.
• The result: NDE1 depletion reduces centrosome integrity, lowers core centriolar protein enrichment, increases paired centriole separation, and disrupts pericentriolar material and focused centrosome-associated microtubule arrays.

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