Analysis of 515 human proteomes defines plasma extracellular vesicle core composition, corona and coisolated networks.

A meta-analysis of 515 human plasma extracellular vesicle proteomes defines a reproducible core proteome, governed by surface-charge-driven mass-action and linked to thrombin-induced platelet degranulation.

• Why it matters: Plasma EV proteomic profiling is confounded by massive co-isolation, making it hard to distinguish true vesicle content from contaminants and biological carryover.
• What they did: The study meta-analyzed 515 EV proteomes and decomposed the core isolate against pan-synthetic coronas and agonist-induced platelet EVs, then assessed residual cargo versus thrombin-induced platelet degranulation.
• The result: The residual vesicular cargo is strongly correlated with thrombin-induced platelet degranulation, and the authors provide a modular atlas to mathematically separate biophysical coronas from cellular co-isolates.

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