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Enhanced cfDNA methylation profiling by detection and correction of jagged ends.
Nature Methods · · Journal Article · Open access
Wang, Hamilton + more
Abstract ↗AI summary
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JEEPERS corrects jagged-end–induced methylation artifacts in cfDNA, preserving duplex integrity and native methylation patterns while enabling high-fidelity tumor burden measurement.
- Why it matters: cfDNA methylation profiling can be confounded by end-repair during double-stranded library preparation, which can create spurious methylation signals.
- What they did: The JEEPERS framework detects and corrects jagged-end–driven methylation errors during duplex sequencing library preparation, then evaluates performance for methylation features and mutation-linked signals.
- The result: After correction, it supports allele-specific methylation differences at recurrent somatic mutations, improves diagnostic performance across liquid biopsy tasks, and enables more precise molecular profiling and disease monitoring.
The findingWhy it mattersWhat they didThe result
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