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OVOL1/2 regulate trophectoderm differentiation during the morula-to-blastocyst transition.
Communications Biology · · Journal Article · Open access
Zhu, Chen + more
Abstract ↗AI summary
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OVOL1/2 are critical ZGA genes in human preimplantation embryos, and their underactivation is linked to blastocyst formation failure after the morula-to-blastocyst transition.
- Why it matters: Most in vitro cultured human embryos arrest at cleavage, but the molecular reasons for impaired blastocyst formation and ZGA failure are not well defined.
- What they did: Single-cell RNA sequencing of biopsied blastomeres from morphologically high-quality 8-cell human embryos was used to track progression, and OVOL1/2 function was tested in both mouse and human embryos.
- The result: Depleting OVOL1/2 disrupts trophectoderm differentiation through ARPC1B-dependent mechanisms, impairing the morula-to-blastocyst transition and providing mechanistic causes of blastocyst formation failure.
The findingWhy it mattersWhat they didThe result
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