Nat BiotechnolJClub
Recordanelles for transcriptome-wide single-cell RNA recording in human cells.
Nature Biotechnology · · Journal Article · Open access
Reinkemeier, Manero-Carranza + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Recordanelles can record up to 90% of endogenous mRNAs in human cells, preserving dynamic transcriptional responses over several days at the single-cell level.
- Why it matters: Understanding transcriptional dynamics is limited by current technologies that only provide snapshots or narrow gene histories, hindering insights into cellular processes over time.
- What they did: The authors engineered a synthetic organelle called the recordanelle, composed of a mitochondrial membrane anchor, condensate assembler, and poly(A)-binding protein, to capture transcriptomes in human cells.
- The result: This technology enables nondisruptive, transcriptome-wide, single-cell RNA recording, allowing detailed study of transient and dynamic gene expression responses over time.
The findingWhy it mattersWhat they didThe result
- Open access