CRISPR-based live-cell DNA imaging: Technologies, biological insights and future perspectives.

Live-cell DNA imaging has advanced rapidly with CRISPR/Cas, enabling real-time visualization of genome behavior across non-repetitive loci and multicolor imaging approaches.

• Why it matters: The field needs direct, dynamic views of how DNA behaves in living cells to connect genome motion with replication, repair, chromatin organization, and regulation.
• What they did: This review summarizes progress in CRISPR-based live-cell DNA imaging, focusing on non-repetitive locus visualization and multicolor imaging, and surveys biological applications.
• The result: The technologies have enabled insights into DNA replication, damage and repair, chromatin organization and interactions, epigenetic regulation, extrachromosomal DNA, and viral genome dynamics, guiding future trends.

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