Promoter engineering in oleaginous yeasts for value-added chemical production.

Promoter engineering is a key strategy for tuning transcriptional programs in oleaginous yeasts like Yarrowia lipolytica to match phase-dependent metabolic states during fermentation.

• Why it matters: Carbon flux shifts across growth, nutrient limitation, lipid accumulation, and production phases, so static constitutive expression is often insufficient for efficient pathway performance.
• What they did: This review summarizes constitutive, inducible, and dynamic promoter systems, links them to the metabolic basis of phase-dependent gene expression demand, and discusses machine learning for promoter prediction and design.
• The result: The work outlines current challenges—limited host-specific datasets, context dependence, and uncertain robustness during scale-up—providing a basis for more precise and scalable engineering of oleaginous yeast cell factories.

AI summary of the abstract · may contain errors · not medical advice · read the paper.