The abstract is read at the publisher; the summary is JClub's.
A compact, high-speed light-sheet fluorescence microscope achieves 850 nm isotropic, diffraction-limited resolution across cleared samples up to 1 cm³ with refractive indices 1.33–1.56.
- Why it matters: High isotropic resolution in centimeter-scale cleared tissues is constrained by slow, axially scanned light sheets that are often aberrated.
- What they did: The system uses off-the-shelf optics with an air objective plus a meniscus lens and an axially swept light sheet for aberration correction, while a concave mirror doubles the effective field of view. Closed-loop feedback on light-sheet motion increases speed tenfold to 100 frames per second.
- The result: Benchmarks across subcellular to centimeter scales and multiple clearing methods show the approach maintains resolution and field of view while enabling rapid imaging.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.