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The periplasmic substrate-binding protein ThuE is required for sucrose uptake and multiplication of Pseudomonas syringae pv. tomato DC3000 in tomato leaves
bioRxiv · · Preprint · Open access
Deng, Kim
Abstract ↗AI summary
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Loss of the periplasmic substrate-binding protein ThuE in Pseudomonas syringae pv. tomato DC3000 reduces bacterial populations in tomato leaves by about two orders of magnitude.
- Why it matters: Sucrose is abundant in the leaf apoplast, but the bacterial steps that limit sucrose acquisition and enable multiplication in planta are unclear.
- What they did: Using base editing, the study created mutants in six candidate sucrose utilization genes in Pto, then tested growth in vitro and during infection of tomato leaves.
- The result: ThuE impairment limits sucrose-dependent growth in both settings, indicating that sucrose import via a non-redundant inner membrane ABC transporter is required for in planta multiplication and may be a control target for bacterial speck disease.
The findingWhy it mattersWhat they didThe result
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