Development of auxin reporters in oilseed rape (Brassica napus)

DR5cc and BIP3 auxin GUS reporters in Brassica napus show auxin-responsive expression in the root apical meristem, with DR5cc giving a more defined pattern than BIP3.

• Why it matters: Species-specific molecular tools are missing for oilseed rape, limiting detailed mapping of auxin distribution and signaling needed to study growth and stress adaptation.
• What they did: The authors built DR5cc and a synthetic BIP3 promoter driving GUS, tested them in hairy roots, transgenic seedlings, flowers at anthesis, and 12-day-old embryos, and used DR5cc fluorescence in hairy roots under mannitol or NaCl.
• The result: In hairy roots, mannitol and NaCl increased DR5cc fluorescence in a time-dependent manner, peaking at 6–12 h and returning to basal by 24 h, and qDII reporters enabled quantitative auxin depletion readouts.

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