The abstract is read at the publisher; the summary is JClub's.
In mouse embryonic stem cells and neural progenitor cells, the promoter-proximal region is necessary for cell-type specific activation of a Sox9 promoter by distal neuronal enhancers.
- Why it matters: Long-range enhancer regulation must selectively activate one target promoter while avoiding other promoters within the same regulatory domain, but the promoter features enabling this specificity were unclear.
- What they did: Using high-throughput genome engineering in mESCs, the endogenous Sox9 promoter was replaced with libraries of core and full promoters, then enhancer-driven responsiveness was tested in mESCs and neural progenitor cells.
- The result: Only a subset of full promoters responded to distal neuronal enhancers; core promoters alone were insufficient, and the promoter-proximal region filtered and transmitted enhancer signals to confer responsiveness.
The findingWhy it mattersWhat they didThe result
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