The abstract is read at the publisher; the summary is JClub's.
BCL11B’s bipartite C2H2 zinc-finger domain binds clustered TG(n)CC(n) DNA motifs with configurational flexibility, including 1-bp-spaced same-orientation TGACC(c/t) pairs.
- Why it matters: Understanding how BCL11B recognizes short, repeated DNA elements is key to explaining its roles in T-cell development and T-cell malignancies.
- What they did: The authors solved eight crystal structures of ZF2-3 bound to 12- or 20-bp DNA duplexes with one or two motifs, and analyzed BCL11B ChIP-seq peaks for motif spacing and orientation.
- The result: ChIP-seq showed strong enrichment of 1-bp-spaced, same-orientation TGACC(c/t) motif pairs, and missense variants at DNA-contacting residues weakened binding affinity/specificity or shifted base recognition preferences.
The findingWhy it mattersWhat they didThe result
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