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Nondisruptive 3D profiling of combinations of epigenetic marks in single cells.
Nature Methods · · Journal Article · Open access
Chen, Radda + more
Abstract ↗AI summary
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Epi-PHR enables nondisruptive, image-based single-cell spatial profiling of combinations of epigenetic marks at hundreds of gene targets while preserving 3D genome organization.
- Why it matters: Spatial single-cell methods that can detect locus-specific epigenetic combinations without disrupting 3D genome structure are still lacking, limiting insight into epigenome organization.
- What they did: The authors developed epigenetic proximity hybridization reaction (Epi-PHR) and used phased Epi-PHR together with chromatin tracing to measure mark enrichment alongside chromatin folding at an imprinted gene cluster.
- The result: In single-chromatin-copy measurements, epigenetic mark enrichment is linked to chromatin folding of the imprinted gene cluster for alleles with distinct parental origins, supporting allele-specific combinatorial epigenetic coding.
The findingWhy it mattersWhat they didThe result
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- Open access
- 5 cites