Comprehensive proteomic profiling reveals mRNA splicing suppression during human oocyte aging.

Splicing machinery is suppressed during human oocyte aging, with FGOs showing protein changes by age 30 and MII oocytes not until 35–40, linked to mRNA splicing, translation, and chromosome segregation.

• Why it matters: Reproductive aging reduces female fertility, but the molecular drivers of oocyte aging and actionable targets remain unclear, limiting effective clinical interventions.
• What they did: The study performed comprehensive proteomic profiling of fully grown germinal vesicle oocytes and metaphase II oocytes across ages 25, 30, 35, and 40, integrating results with transcriptomic data and testing splicing inhibition in mouse and human oocytes.
• The result: Splicing inhibition suppressed mitochondrial function and ROS levels in both species, and DHX15 was identified as a candidate regulator of stage-specific oocyte aging.

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