Research progress in the production, purification, and applications of long single-stranded DNA.

Long single-stranded DNA remains technically challenging to produce, prepare, and purify, and current reviews synthesize strategies spanning biological production, enzymatic amplification, and viral-vector generation.

• Why it matters: Progress is needed because long ssDNA is central to DNA origami, gene therapy, and synthetic biology, yet bottlenecks in manufacturing and purification limit scalable, practical use.
• What they did: The review compares phage-Escherichia coli production, template-dependent enzymatic amplification, and viral-vector generation, then details CRISPR-Cas-assisted post-synthetic processing and other enzymatic strand-conversion methods.
• The result: It outlines purification techniques and application areas, and highlights development trends such as template-independent de novo enzymatic synthesis, automated DNA manufacturing, synthetic-biology production systems, and AI-assisted sequence optimization to support scalable transition.

AI summary of the abstract · may contain errors · not medical advice · read the paper.