CDC48 protein regulates the turnover of peroxisomal catalase in tobacco.

Overexpressing CDC48 in tobacco cell lines markedly lowers peroxisomal catalase CAT1 abundance and activity, with rapid CDC48-dependent degradation of ubiquitinated CAT1.

• Why it matters: Protein homeostasis in plant cells depends on balanced synthesis and degradation, and CDC48’s partner network suggests redox-linked regulation that needed direct substrate identification.
• What they did: The study used tobacco cell lines overexpressing CDC48 to test CAT1 turnover, measuring CAT1 polyubiquitination and degradation while inhibiting deubiquitinating enzymes or CDC48 ATPase activity.
• The result: CDC48 overexpression reduced CAT1 levels and correlated with impaired peroxisomal function, while blocking deubiquitination or CDC48 ATPase activity caused accumulation of ubiquitinated CAT1.

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