Decoder-FFPE-seq enables sensitive, genome-wide spatial transcriptomics of archival tissues at single-cell resolution.

Decoder-FFPE-seq enables single-cell resolution spatial transcriptomics of FFPE tissues with a >2.6-fold sensitivity increase in whole-transcriptome mouse brain and archived human samples.

• Why it matters: High-sensitivity, genome-wide, single-cell spatial RNA profiling from archival FFPE material is still difficult, limiting accessible histopathology and translational discovery.
• What they did: The method uses a dendrimeric DNA coordinate barcoding design with RNA-to-DNA probe conversion and blotting-assisted barcoding to perform FFPE tissue-adapted spatial RNA sequencing.
• The result: It resolves low-abundance gene identities, finer tissue architecture, and captures an epithelial-mesenchymal transition-associated signature, plus long noncoding and viral RNAs in OSCCs.

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