Multiomic proximity labeling in vivo by D-amino-acid-activated peroxidase reaction.

PRADA enables in vivo proximity labeling of proteins and RNAs across multiple organisms, revealing RNA folding as a key regulator of mitochondrial gene expression.

• Why it matters: Understanding the spatial organization of biomolecules in living systems is crucial for deciphering biological functions and regulatory mechanisms, yet remains challenging with existing methods.
• What they did: The study developed PRADA, a D-amino-acid-activated peroxidase reaction, allowing in situ protein and RNA labeling, and applied it in flies, worms, fish, and mice, including a mouse xenograft model.
• The result: PRADA provided detailed insights into mitochondrial proteome, transcriptome, and RNA structure, demonstrating its potential to uncover complex biomolecular organization and regulation in vivo.