The abstract is read at the publisher; the summary is JClub's.
SureCLIP and DrCLIP enable sub-hour protein interaction mapping without genetic manipulation, using reversible crosslinkers DSP and DTBP in cultured cells and wild-type zebrafish.
- Why it matters: Transgene-based proximity labeling is slow and risky in primary or sensitive cells, and zebrafish transgenesis plus overnight labeling cannot capture embryogenesis-scale dynamics.
- What they did: SureCLIP crosslinks proteins with DSP or DTBP and analyzes Hic-5 and lamin B1 in cultured cells; DrCLIP applies the same reversible chemistry for stage-resolved endogenous interactomes in wild-type zebrafish.
- The result: SureCLIP matched published proximity labeling performance in coverage and enrichment while finding new interactors, and DrCLIP revealed kindlin-2 is required for ECM assembly at somite boundaries and myotendinous junction formation.
The findingWhy it mattersWhat they didThe result
AI summary of the abstract · may contain errors · not medical advice · read the paper.