Spatial mapping of RNA turnover kinetics in the mouse brain.

Spatial NT-seq in the mouse brain shows strong regional heterogeneity in RNA turnover, with the dentate gyrus as a hotspot marked by coordinated increases in basal RNA synthesis and decay.

• Why it matters: Measuring RNA degradation and synthesis together across intact tissues is difficult, limiting how well gene regulation can be mapped in vivo.
• What they did: The authors combine transgenesis-free metabolic RNA labeling with in situ chemical recoding on spatial transcriptomics platforms, then apply computational modeling to infer sequence features and post-transcriptional regulators of mRNA stability.
• The result: The method reveals rapid, brain region-specific transcriptional and post-transcriptional responses to electroconvulsive stimulation and enables a spatially resolved view of RNA turnover kinetics and regulatory architecture in vivo.

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