Chromosomal requirements for formation of multivalent human nucleoli.

Deleting an entire NOR-bearing acrocentric p-arm in human cells destroys that chromosome’s nucleolar association potential, showing multivalent nucleoli depend on p-arm context.

• Why it matters: Multivalent human nucleoli involve up to 10 NOR-bearing acrocentric chromosome p-arms, but the chromosomal requirements for their organization were unclear.
• What they did: Using precision megabase-scale chromosome engineering, the study performed NOR deletions and p-arm deletions, including removal of NOR-distal, NOR-proximal, or all p-arm sequences.
• The result: NOR deletions show p-arm clustering can occur independent of rDNA, while individual NOR-distal or NOR-proximal deletions have only a marginal effect on nucleolar association.

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