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Chromosome-Level Reference Genome Assembly Provides Insights Into C21 Steroid Saponins Biosynthesis in Cynanchum atratum.
Plant, Cell & Environment · · Journal Article
Ye, Zhang + more
Abstract ↗AI summary
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A chromosome-level genome assembly for Cynanchum atratum produced a 361.85 Mb assembly with contig N50 5.13 Mb and 86.55% scaffolded into 11 pseudochromosomes.
- Why it matters: C21 steroidal saponins like cynatratoside-C are key medicinal compounds, but the genetic basis for their biosynthesis in C. atratum has been unclear.
- What they did: The team built the assembly from PacBio long reads, annotated 18 871 protein-coding genes (98.3% functionally annotated), then profiled roots, stems, and leaves by transcriptome and LC-MS across 4 months.
- The result: Leaves were the main accumulation tissue of cynatratoside-C, peaking in October in leaves of 2-year-old plants, and metabolite–expression correlations highlighted 11 CYP and 16 UGT genes plus a putative gene cluster on chromosome 3.
The findingWhy it mattersWhat they didThe result
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