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MdPHYB2-MdPIF1 Module Regulates Transcription Factors to Mediate Light-Dependent ALA-Induced Apple Anthocyanin Biosynthesis.
Plant, Cell & Environment · · Journal Article · Open access
Zhang, An + more
Abstract ↗AI summary
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In apple under light, MdPHYB2 promotes anthocyanin accumulation, while MdPIF1 acts as a light-dependent interactive repressor that suppresses key transcription factors.
- Why it matters: Light and 5-aminolevulinic acid (ALA) both increase anthocyanins in apple, but the regulatory link between light perception and ALA-driven transcription was unclear.
- What they did: The study used MdPHYB2 and MdPIF1 overexpression and RNA interference, plus protein interaction assays showing MdPHYB2 and MdPIF1 interact only under light; MdPIF1 directly binds E-box motifs in MdWRKY71, MdMADS1, and MdNAC33 promoters.
- The result: ALA upregulated MdPHYB2 but depressed MdPIF1 under light, enabling nuclear MdPHYB2 to antagonize MdPIF1 promoter binding and thereby alleviate repression to activate anthocyanin biosynthesis.
The findingWhy it mattersWhat they didThe result
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