3D DNA walker-based biosensors: From programmable trajectory to detection of foodborne pathogens.

3D DNA walkers enable highly sensitive biosensors with programmable movement for detecting foodborne pathogens, advancing food safety monitoring.

• Why it matters: Effective detection of foodborne pathogens is crucial for public health, yet current methods lack the sensitivity and programmability offered by 3D DNA walker technology.
• What they did: The review analyzes construction strategies, driving mechanisms, and biosensor designs—covering five key motion control methods and various detection platforms—based on 3D DNA walkers.
• The result: This work highlights the potential of 3D DNA walker-based biosensors to improve rapid, sensitive pathogen detection, supporting large-scale application and technological innovation in food safety.