Flavin-dependent enzymes as multifunctional biocatalysts: A mechanistic perspective.
Flavin-dependent enzymes demonstrate extensive catalytic promiscuity, enabling over 20 distinct non-native reactions through natural and engineered mechanisms.
- Why it matters: Understanding and harnessing this promiscuity is crucial for expanding biocatalysis applications in complex and non-natural chemical synthesis, addressing limitations of traditional catalysts.
- What they did: The review categorizes flavin enzyme reactivity into natural mechanism-based and mechanism-distinct promiscuity, analyzing 6 enzyme families with mechanistic insights and engineering strategies.
- The result: This mechanistic framework guides future enzyme design, enabling the development of highly selective, versatile biocatalysts for innovative chemical transformations.