Nat Chem BiolJClub
Genetic recording and in situ readout of single-cell signaling memory.
Nature Chemical Biology · · Journal Article
Hao, Liu + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
INSCRIBE uses CRISPR base editing to genetically record WNT and BMP signaling in engineered human cells, accurately recovering signal intensity and duration and revealing BMP memory lasting up to 3 weeks.
- Why it matters: Quantifying signal intensity and exposure duration in single cells is difficult, limiting quantitative analysis of how cell-cell communication shapes development and disease.
- What they did: INSCRIBE mutates genomic targets at rates proportional to signaling activity and recovers edits at the endpoint using a ratiometric two-fluorescence-channel imaging readout.
- The result: After defined exogenous stimulations, INSCRIBE accurately recovered intensity in dose-response and duration in time-course experiments, and progeny of high-responding cells stayed more sensitive to BMP for up to 3 weeks.
The findingWhy it mattersWhat they didThe result
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