The abstract is read at the publisher; the summary is JClub's.
Within-host evolution of the metabolically attenuated, epithelial-invasive Salmonella enterica serovar Typhimurium vaccine strain STmAux for up to five years preserves SPI-1 function while BarA/SirA tuning sharply reduces SPI-1ON frequency.
- Why it matters: SPI-1 is a cooperative invasion program that inflammatory models often select to be lost via cheater mutants, but persistent human NTS isolates rarely show obvious SPI-1 loss-of-function, leaving SPI-1’s fitness role unclear.
- What they did: STmAux was evolved within the murine intestine for up to five years, then tested for SPI-1ON dynamics and virulence; parallel evolution repeatedly targeted the BarA/SirA two-component system, and reconstructed early isolates were assayed in vivo.
- The result: Evolved barA and sirA alleles tuned SPI-1 by reducing the proportion of SPI-1ON cells while preserving individual epithelial invasion competence, and early evolved isolates retained full intestinal and systemic virulence in vivo despite strongly reduced SPI-1ON frequencies in vitro.
The findingWhy it mattersWhat they didThe result
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