Brain and kidney Extracellular Vesicle Morphology and Complementary Brain proteomics after Inflammatory Challenge

In rat brain EVs after intracerebroventricular LPS, PTGFRN and LAMB2 show treatment-linked increases of 14.71-fold (q = 0.0365) and 6.23-fold (q = 0.00585).

• Why it matters: EV shape and protein composition can vary independently, and prior brain proteomics attribution and repeated detection reliability were unclear after inflammatory challenge.
• What they did: Rats received intracerebroventricular LPS and an intravenous miRNA-associated EV formulation; brain EVs were profiled by complementary proteomics with independent recalculation of 1,527 eligible protein accessions and a 953-feature quantitative matrix.
• The result: PTGFRN and LAMB2 remained the only significant treatment-versus-LPS findings, with exploratory normalization yielding 16.43-fold and 6.96-fold increases, implying selective shifts in relative protein representation rather than protein copies per EV.

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