Assembly of minimally fragmented metagenomic pangenome graphs

PANIMaSTA constructs minimally fragmented metagenomic pangenome sequence variation graphs for Anelloviridae from over 5,200 diverse genomes, with topology reflecting global ANI similarities.

• Why it matters: Metagenomic pangenome graphs often become highly fragmented into multiple components, which blocks reliable comparisons of genomes embedded across components. This is especially difficult for hypervariable Anelloviridae with short circular genomes.
• What they did: The workflow progressively builds graphs, adding genomes in an order given by a maximum spanning tree from an all-to-all pairwise Anelloviridae ANI similarity matrix.
• The result: The resulting graph topology matches the global genome similarities in the ANI matrix and enables similarity-aware sequence-to-graph mapping and downstream genetic analyses across highly diverse genomes.

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