CyranoPS, a novel compact temperate corynephage infecting Corynebacterium glutamicum MB001

CyranoPS, a temperate corynephage infecting Corynebacterium glutamicum MB001, has a 14,910-bp genome and establishes lysogeny via chromosomal integration.

• Why it matters: Understanding how compact temperate phages switch between lysogeny and lytic growth is limited, especially for Corynebacterium-infecting actinophages with small genomes.
• What they did: The authors characterized CyranoPS by negative-stain electron microscopy and genome analysis, then tested infection using deletions of PorAH and MytC, and assessed lysogeny and superinfection protection.
• The result: Deleting PorAH or MytC abolished infection, while PorBC and ProtX were dispensable; integration truncated Cyr_18 and the truncated repressor gave stronger superinfection protection, with spontaneous induction reaching 109 PFU/mL and no mitomycin C increase.

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