Defining cell type-specific diurnal gene expression in the mouse retina

Single-nucleus RNA sequencing in mouse retina over day and night identifies rhythmic genes in six major retinal cell types, with distinct diurnal patterns across photoreceptors, interneurons, retinal ganglion cells, and Muller glia.

• Why it matters: Daily oscillations in retinal gene expression are linked to retinal physiology and visual function, but how these temporal programs vary by cell type is not fully resolved.
• What they did: The study profiled mouse retina across the light cycle using single-nucleus RNA sequencing, then mapped rhythmic genes in six major retinal cell types and compared diurnal behavior of circadian clock and dopamine pathway genes.
• The result: Core circadian clock genes show differences in paralog expression and rhythmicity across the retina, while dopamine signaling via DRD4 is highly rhythmic across most retinal cell types, enabling exploration of cell-type-specific diurnal rhythms.

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