Analysis of sperm processing and cryopreservation procedures in the common marmoset (Callithrix jacchus) to reduce fragmentation and maximize total and progressive motility

A 30-minute incubation at 38C in TYH media followed by microfluidics yields higher motile and progressively motile common marmoset sperm than swim-up or gradient processing, and supports cryopreservation with at least 20% progressive motility.

• Why it matters: Reliable sperm processing and freezing standards are needed for IVF in the common marmoset, because thermal stress from cryopreservation and inconsistent protocols can impair post-thaw motility.
• What they did: Semen was pre-processed via one of four incubation steps, then processed by swim-up, sucrose gradient, or microfluidics, with sperm quality measured by Hamilton Thorne CASA and DNA fragmentation assays after thawing.
• The result: An optimized cryopreservation protocol using different cryoprotectants and cooling methods consistently achieved at least 20% progressive motility and showed the least significant change in post-thaw motility without DNA damage versus previously published or commercial methods.

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