DeepPhage: leveraging the power of NGS phage display data, an in-depth analysis for the identification of Eu3+-binding peptides

DeepPhage enables comparative analysis of PSD-NGS Eu3+ biopanning datasets by identifying differences in peptide composition across experimental conditions and supporting detection of target-binding candidates.

• Why it matters: PSD-NGS interpretation is hindered by biases from library construction, selection and propagation, plus enrichment of target-unrelated peptides that distort sequence distributions.
• What they did: The tool combines statistical analyses with comprehensive visualization to compare multiple libraries or selection rounds in Eu3+ phage display experiments using PSD-NGS input data.
• The result: DeepPhage provides a reproducible, open-source command-line framework that supports identifying sequence bias, library heterogeneity, and potential target-binding peptides across multiple experiments.

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