Electrically Enhanced Intraperitoneal Drug Delivery Resolved by Fluorescence Lifetime Imaging Microscopy

EMDA with cationic RL-100 NPs co-loaded with doxorubicin enables DOX detection to ~1200 um in a syngeneic rat colorectal peritoneal metastasis model, versus <200 um with passive delivery.

• Why it matters: Intraperitoneal nanoparticle delivery for peritoneal metastases needs proof of intracellular payload release, but autofluorescent tissues make conventional readouts difficult.
• What they did: The study combined electromotive drug administration with RL-100 NPs carrying DOX plus a near-infrared aza-BODIPY tracer, using phasor fluorescence lifetime imaging microscopy to assess DOX’s molecular environment.
• The result: NP-delivered DOX shifted from cytoplasmic puncta to nuclear accumulation while carrier-associated fluorescence stayed cytoplasmic, supporting intracellular release; EMDA also preserved detectable NP-delivered DOX for 24 h.

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