A MultiOmics time-resolved systems map of gene regulation in Toxoplasma gondii

A time-resolved multi-omics map in Toxoplasma gondii links lytic-cycle programs to activating and repressive inputs, organizing genes into 108 co-regulated communities.

• Why it matters: Rapid, asynchronous division and incomplete annotations have limited how invasion-competent states are explained, and single-factor studies miss regulatory partnerships.
• What they did: Acute perturbations of 21 transcriptional and epigenetic factors were profiled with promoter occupancy, chromatin accessibility, and molecular validation on a shared single-cell clock.
• The result: The consensus map distinguishes promoter occupancy from perturbation-linked regulation and nominates regulatory partnerships, confirming MORC/HDAC3 and recovering Cdc5/SNIP1 and DMAP1/EPL1, plus a novel AAF1/AP2XI-5 combination.

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