A portable C-terminal rheostat calibrates RecE/Redα exonuclease activity for enhanced bacterial recombineering

Che9cRecET enables Streptomyces recombineering with kilobase-scale DNA using 50bp homology arms and accurate insertion of 79kb biosynthetic gene clusters.

• Why it matters: Efficient large DNA integration is still limited in Streptomyces and other biotechnologically relevant bacteria, constraining genome engineering and pathway construction.
• What they did: The authors screened actinobacteriophage RecET recombinase pairs, then used structural and biochemical work to show Che9cRecE’s extended C-terminal loop tunes 5'-3' exonuclease activity for recombination.
• The result: The portable C-terminal loop boosts recombineering efficiency across phylogenetically diverse RecE/Redalpha exonucleases, including Escherichia coli Redalpha/RecE and Pseudomonas A.

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