GolgiFold: A Structural Predictome of Human Golgi Protein Complexes

GolgiFold predicts more than 1,800 human Golgi protein pairwise and multisubunit complexes, with many interfaces supported by experimental data and validated contacts.

• Why it matters: Only a subset of Golgi assemblies is defined, limiting understanding of Golgi trafficking, protein processing, and homeostasis; systematic mapping could reveal missing components and regulation.
• What they did: GolgiFold builds a structure-based PPI map by integrating AlphaFold-based Golgi PPI predictions with crosslinking, blue-native proteomics of Golgi organelles, and large-scale in silico screens.
• The result: Biochemical tests confirm newly predicted interfaces, including COG1–COG8 contacts within COG subcomplexes and an unanticipated COPA–RER1 interface, enabling hypothesis generation for diverse Golgi protein classes.

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