In vitro propagation of Okra accessions (Abelmoschus esculentus l. Moench) through Nodal Cuttings

MS medium with 1.5 mg/l BAP plus 2 mg/l Silver nitrate (AgNO3) produced the maximum mean number of shoots from nodal cuttings across three okra accessions (Abelmoschus esculentus).

• Why it matters: Seed propagation is conventional for okra, so a reliable in vitro regeneration method is needed to enable faster multiplication and conservation of diverse accessions.
• What they did: Nodal cuttings from three accessions were cultured on factorial CRD treatments using MS medium with BAP (0.5, 1, 1.5 mg/l) and NAA (0.5, 1, 1.5 mg/l), plus additional supplements; shoot/root initiation, counts, lengths, and acclimatized survival were measured.
• The result: Rooting was maximized on media with 2 mg/l IBA at accession Mandura, while tallest roots occurred without growth regulators at Mandura; survival after one month was highest at Mandura, supporting efficient large-scale multiplication and conservation.

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