Label-free volumetric dynamic atlas of fresh mouse organs using dynamic full-field optical coherence tomography

A mobile D-FFOCT platform builds a volumetric atlas of ten fresh mouse organs, imaging ~1 cm2 in 15 min at 0.75-µm lateral resolution and up to 200 µm depth.

• Why it matters: Label-free, quantitative views of tissue microarchitecture and endogenous intracellular dynamics are needed across organs, with reproducible data to support algorithm development.
• What they did: The team imaged ten organs with D-FFOCT, generating large-area mosaics and 3D reconstructions, comparing results with conventional histology and quantifying dynamic intensity and mean fluctuation frequency across nine organs.
• The result: Organs showed distinct dynamic signatures and heterogeneity, and longitudinal imaging after excision revealed organ-dependent temporal changes in brain, liver, and intestinal tissues, with all raw datasets publicly released.

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