Live Spatial Transcriptomics Enables Longitudinal Tissue Mapping

Live Spatial Transcriptomics (Live-ST) enables longitudinal, transcriptome-wide, spatially resolved mRNA profiling in intact living tissue, including repeated drug-response tracking in patient-derived melanoma.

• Why it matters: Existing spatial transcriptomics mostly yields static snapshots from separate specimens, making it hard to infer molecular dynamics of development, disease progression, and therapy response without direct within-tissue tracking.
• What they did: Live-ST uses electroporation-based intracellular sampling plus spatial barcoding with next-generation sequencing to repeat spatial mRNA measurements in living tissue, validated in patterned 2D cell systems and intact patient-derived melanoma.
• The result: Live-ST preserves tissue viability while enabling repeated, non-destructive spatial gene-expression measurements, including longitudinal mapping of drug-response dynamics within the tumor microenvironment.

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