Injector-based pump-probe serial synchrotron crystallography of CrPhotLOV1 at the MicroMAX beamline

Injector-based pump-probe TR-SSX at MicroMAX resolves CrPhotLOV1 structural dynamics by detecting the FMN-Cys57 thioether adduct across four 0–1850 μs light windows, using a single 2 kHz run.

• Why it matters: Robust delivery, pump–probe synchronization, and reproducible processing are key barriers for time-resolved serial crystallography, especially for reliably interpreting light-driven structural changes.
• What they did: Room-temperature serial datasets for independently measured laser-off dark state and four light-activated windows (0–350, 500–850, 1000–1350, 1500–1850 μs) were collected with JUNGFRAU at 2 kHz, plus an interleaved dark reference.
• The result: Consistent activation levels and reproducible difference Fourier and refined extrapolated electron-density models reveal FMN-Cys57 thioether adduct formation and localized chromophore-pocket rearrangements, matching previously reported CrPhotLOV1 light-state models.

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