IG-28 enables identification and quantification of high-confidence isoforms from large bulk and single-cell full-length cDNA datasets

IG-28 enables fast, low-resource identification and quantification of high-confidence isoforms from large bulk and single-cell full-length cDNA datasets, outperforming other tools.

• Why it matters: Full-length cDNA datasets have grown to billions of reads, exceeding the practical limits of most existing isoform identification methods, especially for single-cell data.
• What they did: IG-28 processes and analyzes large full-length cDNA quickly with minimal computational resources, and it integrates into established scRNA-seq workflows; it was benchmarked on multiple bulk and one complex single-cell full-length cDNA dataset.
• The result: In these benchmarks, IG-28 consistently identified high-confidence public isoforms and outperformed established and newer isoform identification tools, including for single-cell full-length cDNA.

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