Double-Stranded RNA Profiling with Mass Photometry

Mass photometry resolves dsRNA-positive species in mRNA samples using J2 antibody, producing discrete molecular-weight profiles that enable trace impurity characterization.

• Why it matters: Bulk dsRNA assays like sandwich ELISA cannot distinguish dsRNA sub-species, limiting quality control for mRNA therapeutics where immunogenic impurities must be characterized.
• What they did: The study uses MP to detect and characterize antibody/dsRNA complexes, showing ionic strength can interfere with mAb/dsRNA stability and measuring a binding affinity of 1 nM under reproducible parameters. It then applies J2 to resolve dsRNA-positive species by molecular weight, and introduces an uncoated-glass assay that excludes bulk mRNA to profile trace dsRNA at higher loading.
• The result: MP is established as a next-generation analytical platform for simultaneously detecting and characterizing dsRNA byproducts in mRNA samples, including trace levels as antibody-bound species.

AI summary of the abstract · may contain errors · not medical advice · read the paper.