bioRxivJClub
Characterization of intermolecular base pairing using AMT crosslinking in mammalian cells during oxidative stress
bioRxiv · · Preprint · Open access
Liao, Betances + 2 more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
AMT crosslinking combined with RNA pulldown detects intermolecular base pairing in mammalian cells, including U3 snoRNA–pre-18S rRNA, and shows reporter mRNA pairing is sensitive to oxidative stress and G3BP1/2.
- Why it matters: Intermolecular base pairing is important for gene regulation but is poorly characterized in cells, especially during oxidative stress where stress granules and translational repression occur.
- What they did: The authors used AMT crosslinking with RNA pulldown to probe reporter RNAs in vitro and in cellulo under oxidative stress, and tested dependence on G3BP1/2; they also used DMS chemical probing.
- The result: AMT captured reporter RNA base pairing and the U3 snoRNA–pre-18S rRNA interaction in cellulo, while DMS accessibility in non-translated regions was similar in stressed versus unstressed cells and in cells lacking G3BP1/2.
The findingWhy it mattersWhat they didThe result
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- Open access
- 1 cites